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FEATURES OF METABOLIC PROCESSES IN CARP (CYPRINUS CARPIO L.) INFLUENCED BY WASTE WATER FROM LIVESTOCK ENTERPRISES

Автор Доклада: 
Kurbatova I.N., Zakharenko M.O., Tsedyk V.V.
Награда: 
FEATURES OF METABOLIC PROCESSES IN CARP (CYPRINUS CARPIO L.) INFLUENCED BY WASTE WATER FROM LIVESTOCK ENTERPRISES

УДК 639.31.045:591.1/.05:504.05.4.054

 

FEATURES OF METABOLIC PROCESSES IN CARP (CYPRINUS CARPIO L.) INFLUENCED BY WASTE WATER FROM LIVESTOCK ENTERPRISES

 

Inna N. Kurbatova, PhD, Associate professor
Mykola O. Zakharenko, Doctor of science, Professor
Viktoria V. Tsedyk, PhD, Associate professor
NUBiP, Institute of animal science and aquatic bioresources

 

Is clear from research inhibitory effect of waste water livestock enterprises on the reactions of tissue respiration, which leads to activation of anaerobic glycolysis in cells associated with increased activity of LDH, and decreased activity of alkaline phosphatase in the blood of fish.
Key words: Two-year carp, waste water from livestock enterprises, total protein, urea, hemoglobin, LDH, Alkaline phosphatase, AlAT, AsAT

The growing concentration of livestock animals on limited territory, the introduction of industrial technologies for production of livestock products, which includes the use of numerous natural and artificial means of stimulating productivity of animals, antioxidants, means of prevention and treatment of animals, have increased the anthropogenic pressure on natural water bodies, especially on the territories occupied by livestock complexes, farms and processing enterprises.

Presence of waste water of livestock enterprises in the water substantially affects the ecological state of water bodies, changes their hydrochemical composition, cause the rearrangement of flora and fauna, and negatively influence

the body of fish, lowers the quality of fish culture.

In this regard, studying of the complex effects of waste water on carp fish as the main object of aquaculture of inland water bodies, will enable to determine the features of the course of metabolic processes in the body and offer effective ways to prevent their negative impact on aquatic ecosystems.

Research methods.

Studies of the peculiarities of metabolism in fish influenced by waste water from livestock enterprises conducted in 2-year old carp in 40 liters volume aquariums. Different amounts of waste water from pig complex were added to the aquariums. Experiments conducted on five groups of fish, 8 species in each. For fish in the first experimental group, 12.5 ml waste per 40 liters of water were added, the second - 25 ml per 40 liters of water, the third - 50 ml to 40 liters of water, and the fourth - 100 ml to 40 liters of water. The control group of fish was kept in ordinary water.

Research results.

Analysis of chemical composition of waste water which result from operation of pig enterprises and gets into the natural reservoirs used for fishery purposes, including ponds, showed that they contained a variety of end products of organic matter exchange of proteins and amino acids, carbohydrates, hormones and hormone-like compounds, growth stimulators, antioxidants, vitamins, macro-and micronutrients, means of protecting animals from infectious and invasive diseases that impair the organoleptic properties of water and can be toxic to fish [1,2,3,4].

It is found that with increasing content of waste water in aquariums, the blood hemoglobin content in fish increased by 2,8% (second experimental group), 8,3% (third experimental group), 16.0% (fourth research group compared with control).

At the same time, in the first experimental group of fish, where the waste water level in the water tank was minimal, the hemoglobin content in blood was not changed compared with control [5,6].

It is possible to make assumption that the decrease of level of dissolved oxygen in water changes the oxidative processes in different cell compartments and increases the body’s need in it [7,8].

However, in the first experimental group of fish that were kept in water with the lowest waste water concentration, LDH activity of blood plasma was lower by 11,6% compared with similar data in control group of fish.

The decreased activity of alkaline phosphatase by 15,8% (second experimental group), 25.1% (third experimental group), 35.7% (fourth experimental group) compared with control indicates the inhibition of reaction of tissue respiration in the tissues and blood of fish. This pattern is observed as a result of corresponding increase of waste water in water tank and is possibly related to the lowering of hydrolysis processes of macroergic compounds in tissues of fish.

Some features in the exchange of nitrogen compounds that are associated with changes in the intensity of ammonium and ureohenez in tissues are observed in research group fish.

The increase of total protein in blood plasma of carp by 3,5% (first experimental group), 19,5% (second experimental group), 27.5% (third experimental group), and 33,4% (fourth research group ) compared with control in conditions of high concentration of waste water from pig farms in aquariums indicates the intracellular redistribution of proteins in tissues and, is possibly associated with the role of protective proteins of γ-globulin fraction.

 

Conclusion.

Therefore, when different concentrations of waste water from pig farms are added to aquarium water, carp demonstrates hindered reaction of tissue respiration, the processes of anaerobic glycolysis in cells are activated, as evidenced by the increasing LDH activity and decreasing alkaline phosphatase activity in the blood of fish compared with control; hypoxia occurs in organism, which is indicated by growth of hemoglobin in blood of experimental fish; the intensity of ammonium and ureohenez processes in tissues changes, as evidenced by the increasing concentration of urea and increased activity of intracellular ALT, AST enzymes in the blood of fish compared with control.

 

Лiтература:

  1.  Экологическая токсикология и биогеохимия загрязняющих веществ в мировом океане: автореф. дисс. на соискание научн. степени докт. наук / С.А. Патин.– М., 1977. – 52 с. 
  2. The influence of water soluble compounds of ccude oils and their fraction on the ontogenetic development of herring fry / W.W. Kuhnho1d // Ber, Dtsch. wiss, Kommiss, IV Teeresforsch. 1969 V. 20, N 2. – Р. 165-171.
  3. Биологические ресурсы моря и нефтяное загрязнение / О. Г. Миронов. – М., 1972. 105 с.
  4. Токсическое воздействие нитритов на рыб / А. Велдре, М. Роома // Экология. 1990. № 11. С. 7173.
  5. Метаболическая система кислотно-щелочного гомеостаза в организме человека и животных / Д.А. Мельничук // Укр. биохим. журн. 1989. Т. 61, № 3. С. 321.
  6. Адаптивная перестройка энергетического обмена в органах при физиологических воздействиях: автореф. дисс. на соискание научн. степени канд. биол. наук / Косенко Е.А. Пущино,1983. 17 с.
  7. Нарушение энергетического обмена в тканях кролика при аммонийном токсикозе / О.Н. Макаренко, Д.А. Мельничук, Л.В. Скорик //Укр. биохим. журн. 1989. Т. 61, № 6. С. 9498.
  8. Аммиак, его обмен и роль в патологии / Н.Б. Козлов. – М.: Медицина, 1972. 97 с.
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